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MYCOPLASMA ISOLATION FROM CLINICAL AND VETERINARY SAMPLES


Innovation by Mycoplasma Experience scientists has resulted in media which readily support the growth of fastidious mycoplasmas eg. M. hyopneumoniae and M. genitalium.

A wide range of mycoplasmas are cultured at Mycoplasma Experience and other species can be sought as required - for example, the acquisition of SAPO licences enable us to include the specified veterinary pathogens, M. mycoides subsp. capri, M. capricolum subsp. capripneumoniae and M. agalactiae.

Ureaplasma urealyticum, U. diversum, U. canigenitalium and ovine ureaplasmas can be isolated with our ureaplasma liquid and solid media.

See our list of species cultured at Mycoplasma Experience for examples of colonial appearance on Mycoplasma Experience solid media.


CLINICAL ISOLATION

Mycoplasma Experience will examine clinical specimens culturally for mycoplasmas and, where appropriate, identify isolations by disc growth inhibition. Media used are tested for their ability to support low passage strains of M.pneumoniae and U.urealyticum.

All media lots used in the testing of a particular sample are inoculated with low numbers of low-passage strains of the species sought in the sample to confirm that the media, and cultural conditions, would be satisfactory to detect the target organisms.

Isolations are stored at -70°C and typed where possible by specific antisera or can be referred for typing by molecular methods. In isolation surveys, limited biochemical testing is carried out to allow grouping of isolates prior to submission of representative strains to a reference centre for identification.

Samples originating outside the UK and EEC will require importation licences. Several such licences are already held and further applications to SAPO would be made without delay.

Within the UK, sample transportation is an integral part of the service and we would endeavour to extend this to samples from other countries. Pre-packaged containers will be delivered by courier (see packing directions for same day, wet ice or solid carbon dioxide (transportation) for return to our laboratories).

This service extends from single samples to surveys with multiple samplings. Isolation work can be conveniently progressed directly to MIC testing - see Contract Research and Development

Current clinical isolation price list


VETERINARY ISOLATION

Veterinary samples can also be submitted for attempted mycoplasma isolation. Please see our veterinary diagnostics page.

Current Veterinary Isolation Price List