Mycoplasma Experience can identify isolates from our laboratories or referred to us.
Traditionally mycoplasmas and related organisms (mollicutes) were identified by growth inhibition (immunofluorescence or disc growth inhibition (DGI)) with specific antiserum.
Mycoplasma Experience has an historical stock of antisera to major pathogens and cell culture contaminants for DGI and many species can be presumptively identified by characteristic colony morphology, coloured colonies on our selective agars and basic biochemistry.
For isolates that present more of a challenge to identify, we can offer the following Molecular Identification services:
1. High Resolution Melting Curve Analysis (HRMA)
HRMA is a post-PCR technique allowing identification of many mycoplasma species by analysis of their unique melting curve.
2. 16S rDNA Analysis
Sequencing of the 16S rDNA region of mycoplasma allows identification of most species. This is carried out in-house with the sequence data provided by a third party lab.