Storage temperatures -
1. Melt the agar component in a boiling waterbath, allow to cool slightly before placing in a 50°C waterbath.
2. Allow the frozen supplement to thaw at 36°C. Mix gently when completely thawed.
3. Place the supplements in the 50°C waterbath with the agar.
Note: For the agar temperature to fall sufficiently after placing in the 50°C waterbath, allow ~30 minutes for large volumes and ~15 minutes for small volumes.
For the supplement temperature to rise sufficiently after placing in the 50°C waterbath, allow ~10 minutes for large volumes and ~5 minutes for small volumes.
4. Mix the agar and supplement thoroughly whilst avoiding bubbles and distribute 8 ml. amounts into 60 mm petri-
5. When set, dry the plates inverted at 36°C for 30 minutes. Store at 4°Cfor up to 6 weeks. See plate storage and handling
Mycoplasma and Ureaplasma Media 50mL, 100 mL and 500mL
1. Allow the frozen media to thaw at 36°C. Mix gently when completely thawed.
2. The liquid bottles can be inoculated directly with Regulatory Test Organisms, or distributed into smaller volumes in air-
( Liquid media may be stored at 4°C for at least 14 days).