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Storage temperatures - Agar (+4°) Supplement (-20°C)

SOLID MEDIA

1. Melt the agar component in a boiling waterbath, allow to cool slightly before placing in a 50°C waterbath.

2. Allow the frozen supplement to thaw at 36°C. Mix gently when completely thawed.

3. Place the supplements in the 50°C waterbath with the agar.

Note: For the agar temperature to fall sufficiently after placing in the 50°C waterbath, allow ~30 minutes for large volumes and ~15 minutes for small volumes.

  For the supplement temperature to rise sufficiently after placing in the 50°C waterbath, allow ~10 minutes for large volumes and ~5 minutes for small volumes.

4. Mix the agar and supplement thoroughly whilst avoiding bubbles and distribute 8 ml. amounts into 60 mm petri-dishes.

5. When set, dry the plates inverted at 36°C for 30 minutes. Store at 4°C. See plate storage and handling

 

 

 

   

   

 

   







LIQUID MEDIA

Mycoplasma and Ureaplasma Media 100 mL and 500mL

 

1. Allow the frozen media to thaw at 36°C. Mix gently when completely thawed.

2. Distribute into air-tight containers. Store at 4°C.

  (Solid and Liquid media may be stored at 4°C for at least 14 days).

PREPARATION OF MYCOPLASMA EXPERIENCE CONTRACT MEDIA (APPLIES TO BOTH MYCOPLASMA AND UREAPLASMA MEDIA)

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